Magnetic Susceptibility Reduction Method for Magnetically Labeled Immunoassay
Magnetic Susceptibility Reduction Method for Magnetically Labeled Immunoassay
Research Overview
In addition to synthesizing biofunctionalized magnetic nanoparticles for the purpose of magnetically labeling biomolecules, a system to measure the ac magnetic susceptibility of the labeled sample was developed.
When a targeted biomolecule was mixed with magnetic fluid possessing biofunctionalized magnetic nanoparticles, portions of magnetic nanoparticles agglomerated to form clusters due to the association with the targeted biomolecule.
Due to the formation of magnetic clusters, the measured ac magnetic susceptibility reduced. The relationship between the mixed-frequency ac magnetic susceptibility reduction and the amount of the detected biomolecule was established.
Core Detection Principle
This research establishes a novel detection method based on the following mechanism:
- Biofunctionalized Nanoparticles: Magnetic nanoparticles are functionalized with biomolecule-specific recognition elements
- Cluster Formation: When target biomolecules are present, magnetic nanoparticles agglomerate to form clusters through biomolecule association
- Susceptibility Reduction: The formation of magnetic clusters causes a measurable reduction in ac magnetic susceptibility
- Quantitative Relationship: The degree of susceptibility reduction correlates with the amount of target biomolecule present
Detection Process
Step 1: Sample Preparation
Biofunctionalized magnetic nanoparticles are synthesized for magnetically labeling biomolecules.
Step 2: Sample Mixing
Targeted biomolecule is mixed with magnetic fluid containing biofunctionalized magnetic nanoparticles.
Step 3: Cluster Formation
Portions of magnetic nanoparticles agglomerate to form clusters due to association with the targeted biomolecule.
Step 4: Susceptibility Measurement
AC magnetic susceptibility of the labeled sample is measured using the developed measurement system.
Step 5: Quantification
The measured reduction in ac magnetic susceptibility is correlated to the amount of detected biomolecule based on the established relationship.
Key Technical Features
1. Biofunctionalized Magnetic Nanoparticles
Magnetic nanoparticles are synthesized and functionalized with specific bio-recognition elements for targeted biomolecule labeling.
2. AC Magnetic Susceptibility Measurement System
A measurement system was developed specifically to detect the ac magnetic susceptibility of labeled samples.
3. Magnetic Cluster Formation
The detection mechanism relies on the formation of magnetic nanoparticle clusters when they associate with target biomolecules.
4. Susceptibility Reduction Phenomenon
The agglomeration of magnetic nanoparticles into clusters results in a measurable reduction of ac magnetic susceptibility.
5. Quantitative Relationship
A relationship between the mixed-frequency ac magnetic susceptibility reduction and the amount of detected biomolecule was established, enabling quantitative analysis.
Method Advantages
Based on the described mechanism, this magnetic susceptibility reduction method offers the following characteristics:
- Direct Detection: AC magnetic susceptibility measurement provides direct detection of biomolecule binding events
- Cluster-Based Signal: Signal generation through magnetic cluster formation
- Quantitative Analysis: Established relationship enables quantification of biomolecule concentration
- Magnetic Labeling: Uses biofunctionalized magnetic nanoparticles for specific biomolecule targeting
- Mixed-Frequency Detection: Employs mixed-frequency ac magnetic susceptibility measurement
How to Cite This Article
Chin-Yih Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, "Magnetic Susceptibility Reduction Method for Magnetically Labeled Immunoassay", Appl. Phys. Lett., vol. 88, 212512, 2006.
BibTeX:
@article{Hong2006,
title={Magnetic Susceptibility Reduction Method for Magnetically Labeled Immunoassay},
author={Hong, Chin-Yih and Wu, C.C. and Chiu, Y.C. and Yang, S.Y. and Horng, H.E. and Yang, H.C.},
journal={Applied Physics Letters},
volume={88},
pages={212512},
year={2006}
}
Keywords
Magnetic susceptibility reduction • Magnetically labeled immunoassay • Biofunctionalized magnetic nanoparticles • AC magnetic susceptibility • Magnetic clusters • Biomolecule detection • Magnetic nanoparticle agglomeration • Mixed-frequency measurement • Quantitative immunoassay
